優化組織處理流程以進行完整周邊聽覺通路的多尺度影像分析

// An optimized tissue processing pipeline for multiscale imaging of the intact peripheral auditory pathway

本研究開發新型組織處理與成像流程，能完整分析單一標本的周邊聽覺通路，包括耳蝸與耳蝸核｜結合多種成像技術與獨特解剖法，保留耳蝸與耳蝸核連結，實現無組織損失的體積神經成像｜此方法亦支持血管和奈米蛋白質分析，並符合倫理3R原則，提升耳蝸研究的廣泛應用價值。

// This study presents a novel tissue processing and imaging pipeline for analyzing the intact peripheral auditory pathway, including cochlea and cochlear nucleus, in single specimens. The method combines multiple imaging techniques and a unique dissection preserving cochlea-CN connections to enable volumetric neuronal imaging without tissue loss. It also supports vascular and nanoscale protein analyses, using an adapted clearing protocol and validated antibodies for robust labeling. This comprehensive, accessible workflow enhances cochlear research and aligns with ethical 3R principles.

Published 2026年7月21日